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What is ASTM Testing?
ASTM testing refers to the procedures developed by ASTM International (American Society for Testing and Materials) to assess the quality and performance of materials and products across various industries. These standards ensure that products meet safety, reliability, and compliance benchmarks essential for market acceptance.
Key ASTM Standards
Common ASTM Standards
Accugen Laboratories performs a variety of ASTM tests, including:
ASTM C1338: Standard antimicrobial test method for determining fungi resistance of insulation materials and facings.
Sample Requirements:
Triplicate specimens from each test sample are required.
If the test sample is of different construction on each face, prepare triplicate specimens of each face.
The preferred specimen thickness is 0.75 in. (20 mm).
Turn Around Time:
4 to 6 weeks
To purchase a copy of ASTM C1338 test method visit ASTM at astm.org
ASTM D5590: Standard antimicrobial testing method for determining resistance of paint films to fungal defacement.
ASTM D5590 Antimicrobial Testing Overview
This antimicrobial test covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
Custom ASTM D5590 Antimicrobial Testing Laboratory Services
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories is committed to delivering customized solutions. Our expert team of microbiologists collaborates with clients to develop tailored ASTM D5590 testing protocols that address specific antimicrobial evaluation needs.
ASTM D932: Standard antimicrobial testing method for evaluating iron bacteria in water and water-formed deposits.
Custom ASTM D932 Antimicrobial Testing Laboratory Services
Our ASTM D932 test method focuses on the determination of iron bacteria through microscopic examination.
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories is dedicated to providing tailored solutions. Our expert team of microbiologists collaborates with you to develop customized testing protocols that meet your specific antimicrobial testing needs.
ASTM E1153: Standard antimicrobial testing method for efficacy of sanitizers recommended for inanimate non-food contact surfaces.
ASTM E1153 Antimicrobial Testing Overview
The ASTM E1153 test method evaluates the antimicrobial efficacy of sanitizers on pre-cleaned, inanimate, non-porous, non-food contact surfaces. It can also assess one-step cleaner/sanitizer formulations for lightly soiled surfaces.
Custom ASTM E1153 Antimicrobial Testing Laboratory Services
With over 25 years of experience in microbial testing, Accugen Laboratories provides tailored solutions to meet your specific needs. Our expert microbiologists work closely with you to develop effective testing protocols that ensure your sanitizers meet rigorous efficacy standards.
ASTM D2020: Standard antimicrobial testing methods for assessing mildew resistance of paper and paperboard.
Custom ASTM D2020 Antimicrobial Testing Laboratory Services
Our ASTM D2020 test method focuses on the qualitative determination of mildew (fungus) resistance in paper and paperboard, particularly for materials treated to resist fungal growth.
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories is committed to providing tailored solutions. Our expert team of microbiologists collaborates with you to develop customized testing protocols that address your specific antimicrobial testing needs.
ASTM E2149: Standard antimicrobial test method for assessing antimicrobial activity of immobilized agents under dynamic conditions.
ASTM E2149 Antimicrobial Testing Overview
The ASTM E2149 standard test method evaluates the resistance of non-leaching antimicrobial-treated specimens to microbial growth under dynamic contact conditions.
In this method, samples are agitated in dynamic shake flasks using a wrist-action shaker to ensure optimal contact between the bacteria and the treated substrates.
Microbial concentrations in treated and untreated samples are measured at the start (zero hours) and after specified contact times, typically at 1 or 24 hours, as determined by the sponsor.
Organisms: Klebsiella pneumoniae, ATCC No. 4352. Other organisms like Staphylococcus aureus , E.coli may be tested upon request.
Sample Requirements: Between 0.5 and 2.0 grams
Contact time: 1 hr. unless a different contact time is specified
Turn around time: 5 to 8 days
To purchase a copy of ASTM E2149 test method visit ASTM at astm.org
ASTM D3273: Standard antimicrobial testing method for resistance to mold on the surface of interior coatings in an environmental chamber.
Purpose of ASTM D3273 Test
The ASTM D3273 test is designed to evaluate the resistance of interior coatings to fungal defacement, specifically assessing mold growth on their surfaces. This antimicrobial testing method utilizes a controlled environmental chamber to simulate severe interior conditions over a four-week period, allowing for a reproducible evaluation of paint films against mold and mildew.
Test Panels Size for ASTM D3273 Test
12.7 mm (1⁄2 in.) thick, 75 by 100 mm (3 by 4 in.)
Preparation of Test Panels for ASTM D3273 Test
Test Panels for ASTM D3273 Antimicrobial test are prepared in triplicate . Two coats of the antimicrobial test material are applied to both faces and to all edges of the panels allowing 1 day between coats unless otherwise specified.
Turn Around Time for ASTM D3273 Test
Panels are observed each week for 4 weeks.
Custom ASTM D3273 Antimicrobial Testing Laboratory Services
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories is committed to providing customized solutions. Our expert team of microbiologists will collaborate with you to develop tailored testing protocols that meet your specific antimicrobial testing needs.
ASTM E2180: Standard antimicrobial testing method for determining the activity of incorporated antimicrobial agents in polymeric or hydrophobic materials.
Purpose of ASTM E2180 Test
The ASTM E2180 test quantitatively evaluates the antimicrobial effectiveness of agents incorporated into or onto hydrophobic or polymeric surfaces. On such surfaces, it is challenging to achieve uniform microbial contact, which is necessary to assess antimicrobial activity. ASTM E2180 uses an inoculated agar slurry to facilitate intimate contact between microorganisms and the treated surface.
ASTM E2180 Test Method Overview
- An agar slurry is inoculated with a standardized culture of bacteria (e.g. Staphylococcus aureus, Pseudomonas aeruginosa) or other microbes.
- The inoculated agar is spread onto the test material and untreated controls in triplicate.
- After the contact time (typically 24 hours), surviving microbes are recovered by eluting the agar from the substrate into neutralizing broth.
- Serial dilutions are plated, incubated, and colonies counted to determine percent reduction on treated vs untreated samples.
- Exposure times can be extended up to 96 hours for more resistant microbes.
This method can confirm antimicrobial activity, quantify differences between treated and untreated surfaces, and assess long-term durability by testing washed and unwashed samples over time.
Turn Around Time: Eight to ten working days (if commonly tested organisms are tested.)
Sample Requirements: 3.0 x 3.0 cm square samples of the treated and control test materials (
Number of Samples: Since each time point requires 3 test pieces, for example for time 0 hour and 24hours= 3×2 = 6 pieces will be required per organism ordered.
An untreated control sample is also required.
Test Organisms: Staphylococcus aureus ,Pseudomonas aeruginosa or Klebsiella pneumoniae .Other microorganisms such as yeast or fungi may also be tested using this procedure.
To purchase a copy of ASTM E2180 test method visit ASTM at astm.org
ASTM D4300: Standard antimicrobial testing method for adhesive films for their ability to resist fungal growth.
ASTM D4300 Antimicrobial Testing Overview
These antimicrobial testing methods (ASTM D 4300) test the ability of adhesive films to inhibit or support the growth of selected fungal species growing on agar plates by providing means of testing the films on two agar substrates, one of which promotes microbial growth, while the other does not.
Custom ASTM D4300 Antimicrobial Testing Laboratory Services
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories is dedicated to providing customized solutions. Our expert team of microbiologists collaborates closely with clients to develop tailored ASTM D4300 testing protocols that meet their specific antimicrobial evaluation needs.
ASTM E2315: Standard guide for assessment of antimicrobial activity using a time-kill study procedure.
ASTM E2315 Time-Kill Test
This test method measures changes of a population of aerobic microorganisms within a specified time period when tested against antimicrobial test materials. There are multiple options to consider when conducting an ASTM E2315 time-kill testing study. These are important to define when conducting this test in order to ensure that certain variables can be distinguished and standardized. These can include:
- Organism selection and growth (see below for more detail)
- Inoculum preparation (see below for more detail)
- Sampling times
- Temperatures
The ASTM E2315 Time-Kill Testing procedure allows a comparison analysis of tests done under multiple different and varying environments, circumstances, etc.
This test’s main purpose and importance is held in that it can be used to evaluate the decrease of a microbial population of test organisms after being exposed to certain test products and materials.
ASTM E2315 Time-Kill Testing Variants
Organism Preparation
- transfer to growth media
- transfer to agar plates
- transfer to slants
Inoculum Suspension Preparation and Determination of the Microbial Population
- Broth Suspension
- prepared directly from broth
- prepared from dilute broth
- Agar Plate / Slant
- washing of the microbial growth from agar surface
Custom ASTM E2315 Antimicrobial Testing Laboratory Services
With over 25 years of experience in microbial and microbiology testing services, Accugen Laboratories offers customized ASTM E2315 testing solutions. Our expert microbiologists will work closely with you to develop tailored testing protocols that meet your specific antimicrobial evaluation needs.
ASTM G21: Standard practice for determining resistance of synthetic polymeric materials to fungi.
ASTM G21 Test Method Overview
The ASTM G21 test method assesses the resistance of synthetic polymeric materials to fungal growth. This includes a range of materials such as paints, plastics, paper, cardboard, and drywall, which may be in various forms like tubes, rods, films, or sheets.
Synthetic polymeric materials are typically resistant to fungi because they do not provide a carbon source that supports fungal growth. However, other components, such as lubricants and colorants, can promote fungal proliferation on these materials. Understanding the conditions that favor microbial growth is crucial during the production of plastic materials.
The ASTM G21 test determines whether synthetic polymeric materials can resist fungal invasion under various environmental conditions, including different temperatures and humidity levels.
Applications of ASTM G21 Testing
- Assessing Antifungal Activity: Evaluates the antifungal properties of paints and coatings.
- Evaluating Plastic Materials: Tests the antimicrobial effectiveness of PVC piping and plumbing materials.
- Analyzing Construction Materials: Assesses the resistance of synthetic polymer-based construction materials to mold and bacteria.
ASTM G21 Testing Procedure
- Preparation: High concentrations of spore suspensions from five fungal species are prepared.
- Inoculation: Nutrient Salt Agar is poured into sterile Petri dishes, and antimicrobial-treated test specimens are placed on the agar surface.
- Exposure: A composite spore suspension is sprayed onto the agar and test specimens.
- Incubation: The inoculated specimens, along with untreated positive and negative controls, are incubated at 28 to 30°C with over 90% relative humidity for 28 days.
This method is widely used to evaluate the resistance of materials to fungal attacks, providing essential data for manufacturers to ensure product durability and performance.
ASTM G21 Test Sample Requirements:
Three to five pieces of 2 x 2 inch size
The simplest specimen may be a 50 by 50-mm (2 by 2-in.) piece, a 50-mm (2-in.) diameter piece, or a piece (rod or tubing) at least 76 mm (3 in.) long cut from the material to be tested.
Film-forming materials such as coatings may be tested in the form of films at least 50 by 25 mm (2 by 1 in.) in size.
If the specimen is different on two sides, three specimens of each, face up and face down, will be tested upon Sponsor request.
ASTM G21 Turn Around Time:
Test itself is 28 days test. Turn around time for ASTM G21 test is 4 to 5 weeks.
Time Lapse / Growth Progression photos can be provided to complement the report results.
Accugen Laboratories, Inc. provides quality testing services of plastic materials using the ASTM G21 test method and other standardized test methods. Call 630-789-8105 for this and other testing services and to receive personalized and customized price quotes.
How it Works?
Simplify Your Testing with Accugen Laboratories
Submit Your Samples
01
Send Us Your Materials
Provide your synthetic polymer samples (like paints or plastics) in labeled and secure packaging.
Customized Testing Plan
02
Consultation
We’ll discuss your needs and create a tailored testing plan.
Testing Process
03
We Conduct the Tests
Our team performs the ASTM tests, including inoculation and evaluation.
Receive Your Results
04
Get Your Report
You’ll receive a clear report with the results and recommendations.
Where Quality Meets Trust
Customized Testing Solutions
Tailored Testing Services
Accugen Labs specializes in developing customized ASTM testing protocols to meet specific client needs. Our experienced microbiologists collaborate with clients to ensure that testing aligns with industry requirements and product specifications.
Proficiency Testing Programs
Quality Assurance
Accugen Labs participates in proficiency testing programs to evaluate and enhance our testing accuracy. By comparing our results with over 4,400 laboratories worldwide, we ensure that our methods and personnel maintain the highest standards of quality.
Regulatory Compliance
Meeting Industry Standards
Our ASTM testing services help you comply with regulatory requirements, ensuring that your products meet necessary safety and performance standards. This compliance enhances your product’s credibility and marketability in the industry.
Years of Experience
Trusted Clients
Employees
Awards
Reach out to us
To learn more about our analytical microbiology testing services, contact us today.
Email: support@accugenlabs.com
Phone: (639)-789-8105
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